Evaluation of syncytium assay for bovine leukemia virus.
نویسندگان
چکیده
منابع مشابه
Early syncytium formation by bovine leukemia virus.
Bovine leukemia virus (BLV) from either persistently infected bat cells or fetal lamb kidney cells induced rapid syncytium formation in F81 indicator cells. Distinct syncytia were seen within 2 h after inoculation of cells with highly concentrated (500-fold) cell-free BLV preparations and within 4 to 8 h when unconcentrated cell-free BLV preparations were used. Indicator cell densities of 1 x 1...
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The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results ...
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A microplate enzyme-linked immunosorbent assay method was developed for the measurement of bovine immunoglobulin G antibody specific to the envelope antigen (glycoprotein 60) of bovine leukemia virus. The test was then performed on 440 serum samples from dairy cows belonging to herds in which bovine leukemia was suspected or which were leukemia free, and the results were compared with those obt...
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BACKGROUND: Bovine leukemia virus (BLV) is an oncogenic deltaretrovirus causing a persistent lifelong infection of B lymphocytes. In addition to the domestic cattle, the virus can also infect water buffaloes. OBJECTIVES: Recent investigations have demonstrated the increasing prevalence of BLV infection among cattle population in Iran. Large popul...
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The results of examination of 375 bovine serum and 150 bovine milk samples for detection of bovine leukemia virus infection by the immunodiffusion technique were compared with those of an enzyme-linked immunosorbent assay (ELISA). It was concluded that the ELISA is another useful method for the detection of antibodies to bovine leukemia virus in serum and milk. The ELISA provides a quantitative...
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ژورنال
عنوان ژورنال: The Japanese Journal of Veterinary Science
سال: 1980
ISSN: 0021-5295,1881-1442
DOI: 10.1292/jvms1939.42.1